PCR and ELISA detection of cassava mosaic virus in a Congolese cassava landrace

The aim of this study is to diagnosing the cassava mosaic virus in the cuttings of the Congolese cassava landrace Manihot esculenta var. Boma. Symptoms severity was evaluated by visual observation of leaves in field and in greenhouse. The PCR (polymerase chain reaction) and DAS-ELISA tests were used to detect the cassava mosaic virus in this variety. The results showed that the disease index on infected plants were about 3.3 in the field and 2.8 in the greenhouse respectively. The presence of African Cassava Mosaic Virus (ACMV) in samples of this variety was detected by DAS-ELISA. The polymerase chain reaction was well performed for the identification of ACMV and not successful for the Uganda variant of East African Mosaic Virus (EACMV-UG) identification in the leaves samples with or without symptoms.