Effect and mechanism of Solanine on proliferation and apoptosis of Jurkat cells

Objective To explore the effect of proliferation and apoptosis of Solanine on acute T lymphocyte leukemia (T-ALL) Jurkat cells and its mechanism. Methods After treated with different concentrations of Solanine, the proliferation of Jurkat cells was detected by CCK-8 assay, and the effect of Solanine on apoptosis of Jurkat cells were detected by flow cytometry.The expression of Bcl-2 and Bax in Jurkat cells were detected by Western blot, and the expression levels of Bcl-2 mRNA and Bax mRNA were detected by real-time fluorescence quantitative polyme-rase chain reaction. Results CCK-8 assay showed that Solanine significantly inhibited the proliferation of Jurkat cells in a dose-and time-dependent manner. The results of flow cytometry showed that the apoptosis rates of Jurkat cells treated with Solanine for 24 h were (2.40±0.98)%, (28.43±4.86)%, (41.56±1.87)%, respectively, in a dose-dependent manner. Western blot showed that Solanine could increase the expression of Bax and decrease the expression of Bcl-2 in Jurkat cells, and they all were dose-dependent. Conclusion Solanine can significantly inhibit the proliferation and induce apoptosis of Jurkat cells. The mechanism is related to the up-regulation of Bax expression and down-regulation of Bcl-2 expression. Key words: Solanine; Jurkat cell; Proliferation; Apoptosis