Determination of tanshinone II_A,hydroxysafflor yellow A and amygdalin in Xinshule Tablets by HPLC

AIM To establish an HPLC for determining tanshinone ⅡA,hydroxysafflor yellow A and amygdalin in Xinsule Tablets(Salviae miltiorrhizae Radix et Rhizome,Puerariae lobatae Radix,Carthami Flos,Persicae Semen,Curcumae Radix).METHODS Eclipse XDB-C18(5 μm,4.6 mm×150 mm) column was used.Tanshinone ⅡA was determined with the moblie phase of methanol-water(75∶ 25),and detection wavelength of 270nm.Hydroxysafflor yellow A was determined with the moblie phase of methanol-acetontrile-70% phosphoric acid(26∶ 2∶ 72) and detection wavelength of 403 nm.Amygdalin was determined with the moblie phase of methanol-water(20∶ 80) and detection wavelength of 210 nm.RESULTS The linear ranges were 0.008-0.040 μg for tanshinone ⅡA,0.026-0.132 μg for hydroxysafflor yellow A and 0.017-0.084 μg for amygdalin.Their average recoveries were 97.61%,98.04% and 98.44%,respectively.Their RSDs were 0.78%,1.09% and 0.82%,respectively.CONCLUSION The method is rapid,accurate,reproducible,stable and has high recovery.It can be used for quality control of Xinshule Tablets.