Moulds Presence on Indigenous Grape Varieties from Miniş-Măderat Vineyard

Abstract Moulds are filamentous fungi which induce alteration on food and feed. The early detection of alteration fungal microbiota is essential for effective control strategies. In this study, we have investigated the moulds diversity of grape berry during bunch closure stage. Fungal strains were isolated from two indigenous grape varieties Cadarcă and Mustoasă of Măderat collected from Minis-Măderat vineyard. The obtained isolates were identified at genus and/or species level by taxonomic criteria. During bunch closure stage, the level of moulds isolates has been 10 2 -10 3 CFU/g. The most frequently isolated filamentous fungus (about 30%) has been identified as Cladosporium sp. In the case of Aspergillus sp. it has been isolated only from Mustoasă of Măderat variety. These results of the classical morphological identification will be further compared with the results of molecular identification by PCR-ITS-RFLP. This study is a part in the first exhaustive study of the fungal diversity of grapes in consecrated Romanian vineyards.