Vitamin D3 increased intestinal Na/Pi-IIb and CYP27B1 mRNA level in rats fed low-phosphorus diets

Abstract The objective of the study was to determine the role of vitamin D 3 (VD 3 ) in regulating adaptation and mechanism of rats to low-phosphorus (P) diets. Rats were assigned to 4 diets containing 0.2%, 0.4%, 0.6%, or 0.8% P consisting of 5 replicate cages with 6 rats per replicate cage and fed for 7 days. Four rats from each replicate cage were treated with ethane-1-hydroxy-1,1-diphosphonicacid, tetrasodium salt (EHDP) and 2 rats remained untreated. Twelve hours prior to preparation on d 7, two of the EHDP-treated rats received an intraperitoneal injection of VD 3 [1,25-(OH) 2 D 3 ] at 600 ng per kg body weight, while two rats did not receive the injection. Rats that did not receive VD 3 injection had decreased ( P 3 resulted in increased ( P 3 injection was greater ( P Na/Pi-II b ) and 25-hydroxyvitamin D 1-α hydroxylase ( CYP27B1 ) mRNA level showed the same trend with P absorption. Serum concentration of VD 3 and 1α-hydroxylase activity in rats fed 0.2% P diet were lower than those fed 0.8% P diet. The injection of VD 3 increased ( P 3 and 1α-hydroxylase activity. Thus, VD 3 increased Na/Pi–IIb and CYP27B1 mRNA level and improved serum concentration of VD 3 and 1α-hydroxylase activity in rats fed low-P diets.