A heat-digestible cell-immunoadsorbent made by coupling hapten to gelatin

Abstract A simple method is described for the separation of mouse splenocytes having receptors specific for the dinitrophenyl (DNP) determinant. The method utilizes the capacity of an immunoadsorbent DNP—gelatin to retain specifically 60 to 90 percent of cells having receptors for DNP. The retained cells can be brought into solution by melting DNP—gelatin at 37°C. Further treatment with collagenase removes the melted DNP—gelatin from the cells. Approximately 95% of the cells remained viable after melting as proved by vital staining and MIF production. Receptor-bearing cells were assayed by using the technique of DNP-coupled bacteriophage.