Determination of trace carbendazim in citrus by high performance liquid chromatography with on-line clean-up and solid phase extraction

Objective To establish a method for determination of carbendazim in citrus by high performance liquid chromatography with on-line clean-up and solid phase extraction. Methods After the samples were purified and enriched in the solid phase extraction column Cyclone-MCX, the solid phase extraction column switch to the analysis flow path by switching valve, then let the target analytes flush from the enrichment column to the analytical column PAⅡC18, accomplished separation and detection of carbendazim by using the method of gradient elution. Results The linear range was 1~20 μg/L, a linear regression equation was fitted as follows: Y=0.0921X-0.0016 with a correlation coefficient of 0.9995. The detection limit was 0.1 μg/L. Average recovery at various levels were 86.5%~96.7% with RSD 2.34%~3.21%(n=6). Conclusion The established method is simple, rapid, accurate and credible, and can be applied in rapid determination of trace carbendazim in citrus with satisfactory results.