The PCR Revolution: The road from qualitative to quantitative assay: What is next?

INTRODUCTION 110 PRE-PCR STEPS 111 INVENTIONS MADE IN “ABSOLUTE” QUANTIFICATION ASSAYS 112 HOW THE RELATIVE QUANTIFICATION STRATEGY CHANGED 114 WHAT ABOUT PCR EFFICIENCY? 115 ASSAY VARIANCE AND HOW TO PERFORM A PROPER NORMALIZATION 116 EXPRESSION PROFILING, qPCR BIOINFORMATICS, AND STATISTICAL ANALYSIS 117 WHAT IS NEXT IN REAL-TIME PCR? 118 GENE EXPRESSION AND MORE – THE SYSTEM BIOLOGY IDEA 120 CONCLUSION 124 REFERENCES 124