Estudio de sobrevida y cultivo de células cocoides en cepas de: Campylobacter jejuni

Campylobacter jejuni, uno de los principales agentes causales de sindrome diarreico en seres humanos, es una bacteria que a bajas temperaturas y en condiciones de estres forma celulas cocoides no cultivables. Para determinar las condiciones en las que cepas de C. jejuni se conserven viables y cultivables independiente de la morfologia, se sometio a dos cepas de este a tres diversas condiciones de laboratorio evaluando morfologia mediante tincion de Gram, viabilidad celular mediante pruebas bioquimicas y Citometria de Flujo. Ambas cepas lograron mantenerse viables y cultivables en dichas condiciones, tanto en la forma bacilar como en la cocoide, siendo la condicion mas desfavorable para ambas cepas la temperatura ambiente (sobrevida de 4 dias y 6 dias), y mas favorable la bolsa hermetica a 4° C (15 Y 22 dias de sobrevida). La citometria de flujo resulto ser una buena herramienta para medir viabilidad de las cepas ensayadas, mostrando que las celulas cocoides presentaban alteraciones en la continuidad de su membrana, indicativo de perdida de viabilidad celular. (AU) Campylobacter jejuni, a major causative agent of diarrhoeal syndrome in humans, is a bacterium that at low temperatures and under conditions of stress, coccoid cells form non-arable. To determine the conditions under which strains of C. jejuni is kept viable and cultivable, independent of the morphology, underwent two strains of C. jejuni three different laboratory conditions, and morphology was assessed by Gram stain, and cell viability by biochemical tests and flow cytometry. Both strains were able to remain viable and culturable in the three laboratory conditions, both in the form of bacilli in the coccoid form, being the most unfavourable condition for both strains, room temperature (survival of 4 days and 6 days), and the most favourable airtight bag 4 0 C (75 and 22 days of survival). The flow cytometry proved to be a good tool for measuring viability of the strains tested, showing that cells coccoid, showed alterations in the continuity of its membrane, indicative of loss of cell viability. (AU)