Electropermeabilization of Isolated Cancer Stem Cells with a Novel and Versatile Nanosecond Pulse Generator

This paper presents the use of a novel electro-poration generator with an artificial 50Ω buffer for possible real-time neutralization of cancer stem cells. The results from an initial bench study investigates the development of an electro-poration generator capable of delivering non-thermal treatment with an original cuvette housing unit and its effects on isolated cancer stem cell. Initial permeabilization investigation of cancer stem cells were conducted, indicating that the developed protocols and devices have a strong potential future use in achieving electro-manipulation of cancer stem cells.