Simultaneous estimation of gliclazide and lacidipine using spectrophotometric methods and its application in nanostructured lipid carrier (NLC) development

Simple, accurate, and precise methods for simultaneous estimation of Gliclazide (Gld) and Lacidipine (Ldpn) in combined-dosage form have been described. Techniques used employ 1st order derivative and simultaneous equationmethod for the simultaneous estimation of the drugs in combination dosage form. Linearity was observed in the concentration range of 2-20 μg/mL for Gldand 4-18 μg/mL for Ldpn. The accuracy of methods was assessed by recovery studies and was found to be within a range of 98-102% for both Gld and Ldpn. The developed methods were validated with respect to linearity, accuracy (recovery), and precision. The results were validated statistically as per ICH Q2 R1 guideline and were found to be satisfactory. The proposed methods were successfully applied for the determination of Gld and Ldpn in the NLC formulation.