EVALUATION OF THE PROTEOLYTIC ACTIVITY PRESENT IN CHO CELL CULTURE SUPERNATANTS

The heterogeneity of recombinant glycoproteins expressed by CHO cells may partly result from a proteolytic activity present in cell culture supernatants. This study investigated the presence of proteases in CHO cell culture supernatants and their effects on the quality of a recombinant secreted viral envelope glycoprotein used as a model.