Determinación de bombesina en estómago de cerdos con diagnóstico de gastritis provocadas por Helicobacter spp

El objetivo del estudio fue analizar la relacion entre Helicobacter spp y la funcionalidad de las celulas productoras de bombesina en biopsias gastricas de cerdos. Se determino la presencia de Helicobacter spp en muestras gastricas de region antro-pilorica de cerdos mestizos. Fueron coloreadas con Hematoxilina/Eosina para el diagnostico de gastritis, observandose mayor proporcion de lesiones cronicas que agudas. La presencia de Helicobacter se realizo mediante tincion Giemsa y Warthin-Starry, determinandose la presencia de al menos dos especies colonizando el estomago del cerdo: una de mayor tamano, intensamente espiralada (Helicobacter heilmannii) y otra de menor tamano, con forma de “s” italica (Helicobacter pylori). Mediante inmunohistoquimica se localizo Helicobacter spp en el epitelio, foseta y glandula gastrica. La deteccion de celulas productoras de bombesina se realizo por tecnicas inmunohistoquimicas y se determino su porcentaje en muestras con gastritis agudas y cronicas, Helicobacter (+) / (-). Las celulas bombesina (+) se localizaron en el epitelio glandular, tejido conectivo subyacente y tejido muscular. Se observaron diferencias estadisticamente significativas entre los grupos: Gastritis Cronica Helicobacter (+) / Gastritis Aguda Helicobacter (+) (p=0,04); Gastritis Cronica Helicobacter (+) / Mucosa Normal (p=0,02) y en Gastritis Cronica Helicobacter (-) / Mucosa Normal (p=0,005). El grupo con mayor numero de celulas bombesina positivas fue el de Gastritis Aguda Helicobacter (+) cuya morfologia celular fue de tipo cerrada.   Bombesin determination in pig’s stomach with gastritis diagnose caused by Helicobacter spp. The objective of this study was to analyze the relationship between Helicobacter spp and the functionality of bombesin-producing cells in gastric biopsies of pigs. The presence of Helicobacter spp was determined in gastric samples from the antro-pyloric region of mixed-breed pigs. They were colored with Hematoxylin/Eosin for the diagnosis of gastritis, finding a higher proportion of chronic than acute lesions. The presence of Helicobacter was carried out by means of Giemsa and Warthin-Starry staining, determining the presence of, at least, two species colonizing the stomach of the pig: one of larger size, intensely spiral Helicobacter heilmannii); and another of smaller size, in the form of “s” italic (Helicobacter pylori). Helicobacter spp was localized in the epithelium, fossa and gastric glands by immunohistochemistry. The detection of bombesin producing cells was performed by immunohistochemical techniques and its percentage was determined in samples with acute and chronic gastritis, Helicobacter (+) / (-). These cells were in the glandular epithelium, underlying connective tissue and muscle tissue. Statistically significant difference was observed between the groups: Chronic Gastritis Helicobacter (+) / Acute Gastritis Helicobacter (+) (p = 0.04); Chronic Gastritis Helicobacter (+) / Normal Mucosa (p = 0.02) and in Chronic Gastritis Helicobacter (-) / Normal Mucosa (p = 0.005). The group with the highest number of bombesin positive cells was that of Gastritis Acute Helicobacter (+), whose cell morphology was closed type.