Survival and differentiation of extrinsic neural stem cells being transplanted into goats' brain cortices

Objective To research the survival and differentiation of neural stem cells being transplanted into goats' brain cortices.Methods We transplanted the neural stem cells marked by green fluorescence protein (GFP) or saline into the surrounding areas of brain contusion and laceration areas of goat's models by surgical intervention.Then,the survival and differentiation of neural stem cells being transplanted into goats' brain were observed one month later with the method of immtmohistochemistry by detecting the specific markers of neurons,astrocytes and oligodendrocytes:microtubule-associated protein 2 (MAP2),glial fibrillary acidic protein (GFAP) and oligodendrocyte marker protein 01 (O1).Results Neural stem cells survived well in the goats' brain,and differentiated into three types of cells:neurons,astrocytes and oligodendrcytes; and these cells mainly expressed MAP2 and GFAP.Conclusion Neural stem cells can survival,immigrate and different well in the transplanted brains. Key words: Neural stem cell;  Cell transplantation;  Survival;  Differentiation;  Goat