Application of principal component regression to luminescence data for the screening of ciprofloxacin and enrofloxacin in animal tissues
2000
A new screening method for the analysis of enrofloxacin and
ciprofloxacin in edible animal tissues is described. The method is based on
the application of principal component regression to luminescence
measurements after reaction of quinolones with terbium(III) in a
micellar medium. The method was used, first, to discriminate between
quinolone-containing or quinolone-not-containing samples (concentration
below the detection limit) and, then, to quantify the sum of both analytes.
Standards in a pure-water matrix, using the first three principal
components, were used for the determination. RRMSE range from 4 to 10%
depending on the analyte. Calibration was successfully applied to the
analysis of spiked chicken and trout muscle at concentrations between 10
and 50 μg kg−1.
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