Novel Mixed Hydrotropic Solubalisation technique for Analytical method development and validation of Acyclovir in bulk and tablet formulation by Spectrophotometry

In pharmaceutical field, it is often required to prepare aqueous solutions of a variety of insoluble drugs. The ability to increase the aqueous solubility can be a valuable aid for increasing the efficacy and/or reducing adverse effects for certain drugs. In the given study the aqueous solubility of acyclovir was found to be 2 mg/ml that give the satisfactory result with regression coefficient of about 0.991. The present study was perfomed on UVspectrophotometer with selected wavelength of 339nm. Linearity range was 10–50 μg/ml) and assay results was found to be 101.48%. The proposed method was new, simple, cost effective, accurate, sensitive, free from pollution and precise and can be adopted for routine analysis of Acyclovir in tablet dosage form. Presence of hydrotropic agent do not shows any significant interference in the spectrophotometric assay thus further confirming the applicability and reproducibility of the developed method.