The cloning and characterization of CYP4A11 gene promoter region

Objective Cytochrome P450 4A11(CYP4A11)may play an important role in the regu- lation of blood pressure via its metabolites 19/20-Hydroxyeicosatetraenoic acids(20-HETE)and the present study identified the promoter sequence and 5'-flanking region of CYP4A11 gene and investigated the molecu- lar mechanisms through which regulates the expression of CYP4A11 gene.Method To functional analysis of CYP4A11 promoter in HepG2 cells,Luciferase reporter plasmids containing vector alone(pGL3-Basic) and serially truncated putative CYP4A11 promoter fragments(1641bp-536kb)which were clone based on the prediction of online-software of UCSC,were transiently transfected into HepG2 cells.Furthermore,we investigated potential transcription factor binding sites in the region fucfions as an enhancer using the predic- tion programs Transfao4.0.Results The highest level of CYP4A11 promoter activity was exhibited by the- 972bp promoter construct.Reducing the promoter length by 252 bp(-720bp promoter construct) and further 184 bp(-536bp promoter construct) caused a significant decrease in activity,by 70.1% and 96.9% in HepG2 cells,respectively(P0.001).Conclution These data indicate that positive regulatory elements may locate in the regions from-972 to-536hp of the CYP4A11 gene.