Generation and Identification of the Monoclonal Antibody Against Fumonisin B_1

【Objective】 Mycotoxins can lead to serious food security issues.This study focused on the generation of the specific monoclonal antibody(McAb) against fumonisin B1.【Method】 The artificial antigen of fumonisin B1 was prepared and a hybridoma cell line was screened with method of hybridoma.Monoclonal antibody was generated by murine ascites and identified.【Result】 A hybridoma cell line of F3 was screened.The antibody secreted by F3 was classified into IgG1 and the light chain type was κ;the results of 10% SDS-PAGE showed that the molecular weight of high chain was 50 kD and the light chain was 25 kD,respectively.The titer of McAb was 1:3 200 in supernatant of F3 and 1:51 200 in ascites with indirect enzyme-linked immunosorbent assay.The affinity was 1.60×10-8 mol?L-1 and the value of IC50 was 3.589 ng?mL-1.There were no cross reactions to other mycotoxins.The high specificity of antibody was identified by western blotting.【Conclusion】The McAb against FB1 in this experiment are of sensitivity and specificity.