Fluorimetric method forsimultaneous estimation of cortisol, corticosterone, andtestosterone inplasma

ofExeter SUMMARY Thesimultaneous estimation ofsteroids inplasma was carried outbytheassayof cortisol, corticosterone, andtestosterone. Themethodentails separation bymeans ofthinlayer chromatography, followed byconversion toafluorophore andfluorimetric measurement. Itsmajor advantages areits high specificity, its ability todetect unknownsubstances, andtheeasewithwhichit canbeperformed. Themethod hasacceptable levels ofaccuracyandprecision andthenormal values obtained byitcomparewell withthose given bymethods ingeneral use.