The High Performance Liquid Chromatography Determines in Pre-mixture Vitamin E content

Establishes one kind to determine Vitamin E with the High Performance Liquid Chromatography the method.Method,Chromatographic column for PRONTOSIL 120-5C18-ace-APS 4.6mm×250mm,5.0μm,flows: Methyl alcohol,speed of flow 1.0mL/Min,ultraviolet detector wave length 285nm,column warm,Normal temperature. Finally,Vitamin E acetate content in 0.0342～0.342mg/mL has the good linear relations,the average returns-ratio is 101.87%,accuracy RSD is 1.5%.Conclusion,This method simple,is rapid,uses in the pre-mixture the VE determination,obtain satisfaction the result.