Self-organization of human embryonic stem cells on micropatterns

Alessia Deglincerti,Fred Etoc,M Cecilia Guerra,Iain Martyn,Jakob J. Metzger,Albert Ruzo,Mijo Simunovic,Anna Yoney,Ali H. Brivanlou,Eric D. Siggia,Aryeh Warmflash

Self-organization of human embryonic stem cells on micropatterns

2016

Alessia Deglincerti
Fred Etoc
M Cecilia Guerra
Iain Martyn
Jakob J. Metzger
Albert Ruzo
Mijo Simunovic
Anna Yoney
Ali H. Brivanlou
Eric D. Siggia
Aryeh Warmflash

This protocol describes how to differentiate and image human embryonic stem cells on micropatterned colonies to create radially organized domains of the germ layers mimicking embryonic gastrulation in vitro.

Keywords:

Endothelial stem cell
Molecular biology
Cell biology
Adult stem cell
Cellular differentiation
Biology
Stem cell
Germ layer
Embryoid body
Embryonic stem cell
Cell culture
Cell type

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