Synthesis and analysis of [3H] salmon calcitonin ([3H]SMC021)

Tritium-labelled salmon calcitonin was synthesized successfully through a 5 step peptide synthesis. LCMS performed on each step played an important role for in process control. For accuracy, Q-TOF MS was used to determine the specific activity of tritiated salmon calcitonin ([ 3 H]SMC021) using the triple charged ion.