Isolation of recombinant hirudin by preparative high-performance liquid chromatography

Abstract The purification of recombinant hirudin varaint 2-Lys 47 (rHV2-Lys 47 ), produced by a genetically engineered yeast strain, is described. rHV2-Lys 47 expressed and secreted into the culture medium was the starting material for the purification process of hirudin from the culture broth aftr cell harvesting by centrifugation. Initial purification of the product by preparative reversed-phase high-performance liquid chromatography (HPLC) using step-gradient elution, followed by precipitation of rHV2-Lys 47 in the presence of acetone, removed most of the contaminants from the culture medium. The pure product was obtained by successive preparative anion-exchange and reversed-phase HPLC on silica based stationary phases. Characterization of the final product by analytical HPLC, isoelectric focusing gel electrophoresis, quantitative amino acid composition and sequence analysis did not reveal any contaminants. Liquid secondary ion mass spectrometry was used to confirm its primary structure. The isolated product was tested in an inhibition assay of human α-thrombin and proved to be fully active.