Comparison of the enzyme dot hybridization assay with double antibody sandwich assay in the detection of Hantavirus infection

Peroxidase horseradish(HRP)-labeled single chain fragment of variable region(scFv) against Hantavirus nucleocapsid protein(NP) antigen was applied in enzyme dot hybridization assay and double antibody sandwich assay to detect Hantavirus infection in order to develop the cheap diagnostic reagents for the early diagnosis of haemorrhagic fever with renal syndrome(HFRS).In these assays,the scFv against Hantavirus NP antigen was conjugated with HRP b glutaraldehyde to prepare the HRP-labeled scFv and the HRP-labeled scFv was then used in enzyme dot hybridization and double antibody sandwich assay to detect Hantavirus infection samples of patients with early HFRS and 66 sera of healthy individuals as control.It was found that using enzyme dot hybridization assay,the positive detection rate was 50.00%(28/29),while that using double antibody sandwich assay is 51.78(29/56),the serum samples of the 66 control individuals showed negative results by using both assays.The results were dealt with statistical methods and the difference in both methods was not obvious.It is concluded that the method of assays using-labeled scFv against Hantavirus NP antigen and applied in enzyme dot hybridization method and double antibody sandwich method are proved to be stable,cheap and reliable with very low false positive rate.