Phosphorus uptake, distribution and transformation with Chlorella vulgaris under different trophic modes.

Abstract Phosphorus (P) uptake, distribution and transformation are important processes associated with the growth and metabolism of microalgae. In this study, the fate of P in soluble microbial products (SMP), extracellular polymeric substances (EPS), and intracellular polymeric substances (IPS) of Chlorella vulgaris C7 in the form of inorganic P (IP) and organic P (OP) was studied under different trophic modes, including photoautotrophy, heterotrophy and mixotrophy. The results showed that mixotrophic cultivation of microalgae brought highest biomass yield, which was 2.09 times and 11.10 times higher than that of the photoautotrophic and heterotrophic conditions. Regarding P distribution and transformation, the trophic modes affected the transformation trends and rates of P in the form of IP and OP among SMP, EPS and IPS. Under photoautotrophic condition, EPS was the main P pool, and most of P in C. vulgaris was IP. While, under mixotrophic condition, IPS was the main P pool, and most of P was transformed to OP. The addition of glucose promoted the uptake of P by algal cells and the transformation of IP to OP, which accounted for 79.32% of total phosphorus at the end of cultivation. Collectively, mixotrophic cultivation was regarded as the optimum approach for microalgae cultivation and nutrient recovery.