Partial suppression of BtpB toxicity by overexpression of yeast genes.

(A) Ten-fold serial dilution assay of yeast cells co-expressing BtpB and each of the seven suppressor ORFs isolated from a yeast genetic screen. pYES3 and pYES2 are the corresponding empty vectors for BtpB and for the overexpressed genes, respectively. (B) Western blotting of W303-1A yeast strain co-expressing GFP-BtpB and each of the proteins encoded by the suppressor genes. Antibodies anti-GFP to detect GFP-BtpB (upper panel) and Anti-G6PDH as loading control (lower panel) were used. Anti-GFP antibody allows the detection of the indicated protein A-tagged proteins due to affinity of the tag with the Fc region of IgG-type antibodies. (C) and (D) Ten-fold serial dilution assays of yeast cells co-expressing BtpB-TIR (C) or BtpA-TIR (D) and the suppressor genes. pYES3 and pYES2 are the corresponding empty vectors for BtpB- or BtpA-TIR and for the overexpressed genes, respectively. (PDF)