Effect of integrin α2 on in vetro expression of MMP-2 of osteosarcoma cell

Objective To study the relationship between cell surface integrin receptor(a2) and matrixmetalloproteinases 2(MMP-2) of osteosarcoma in vivo.Methods After cultured respectively in DMEM media containing 10,25 and 50 mmol/L α2 integrin monoclonal antibody for 6 h,the RNA of MG63 cells was extracted using Trizol assay and mRNA was detected by semi-quantity RT-PCR.After cultured in DMEM media containing 10,25 and 50 mmol/L α2 integrin monoclonal antibody respectively for 48 h,immunohistochemisty assay was used to detect the MMP-2 protein expression.Results With the increasement of the concentrations of α2 integrin monoclonal antibody,the expressions of MG63 mRNA and protein were both down-regulated.Conclusion The α2 integrin acceptor on osteosarcoma cell surface may promote MMP-2 expression on the level of RNA and protein.