The clinical significance of detecting DNA contents and proliferation activity of myeloid cells in acute lymphocytic leukemia.

Objective To investigate the clinical significance of DNA detection on diagnosis and therapy in acute lymphocytic leukemia(ALL). Methods The DNA index(DI)and proliferation index(PI) in aspirated myeloid cells were determinated in 35 patients with ALL by flow cytometry(FCM). Results The aberrant DNA aneuploid was found in 51 4% of the ALL patients examined(18/35), which included 8 hypodiploid and 10 hyperdiploid. In the ALL groups G0/G1% were higher than that of the control( P 0 05)whereas S% and PI were much lower than that of the control( P 0 05). Conclusion DNA detection and ploid analysis are very meaningful criteria both clinically and biologically on diagnosis and therapy of ALL. It contributes to diagnosing ALL exactly and objectively and may become the important parameters for evaluating therapeutic effect and prognosis