OPTIMIZACIÓN DE LAS CONDICIONES ANALÍTICAS IDEALES PARA CUANTIFICAR ALUMINIO EN TEJIDOS DE RATAS SPRAGUE DAWLEY MEDIANTE LA TÉCNICA DE ABSORCIÓN ATÓMICA

The ideal analytical conditions for the quantification of aluminum in tissue of Sprague Dawley rats by using flame atomic absorption spectroscopy (FAAS) were evaluated. The significance of studies focusing on the effect of aluminum (Al) in tissue is based on recent findings that relate Al to brain, liver, hematologic, and neoplasic conditions; and consequently, Al is now rendered as being potentially toxic. Nevertheless, these studies are not conclusive and many of Al possible effects are still ignored. The quality parameters used in this work were: linearity, accuracy (recovery percentage), precision (variation coefficient percentage), and detection (DL) and quantification limits (QL). Tissue samples of Sprague Dawley rats were digested (breast, viscera and bone), along with water and food samples. Al samples were fortified by adding 100 mg/L of Al. In addition, a certified reference material that contains 700 ± 4 mg/L of Al was used (CRM-S-B) to assess the accuracy and precision of the analytical method. The value for linearity, as obtained from the use of Al calibration curves, was r = 0.9998. The accuracy level, measured as a recovery percentage using Al concentrations of 20, 50 and 100 mg/L, was 101, 98 and 97 %, respectively. Precision values, quantified as the coefficient variation percentage (% CV) were 2.15, 1.05 and 1.16 %, respectively. The obtained accuracy and precision results are considered acceptable as the acceptance criterion using instrumental methods is 100 ± 15% for the former and CV≤ 2 % for the later. The concentration levels obtained for DL and QL, using a high-purity standard, were of 0.06 mg/L and 0.19 mg/L, respectively; these values are similar to those reported in the scientific literature. The accuracy results (% of recovery) obtained for Sprague Dawley rat samples were: water (103 %), food (99.3%), breast cancer (100.9 %), viscera (103.5%) and bone (100.6 %). The 100.62 % recovery obtained for the certified reference material CRM-S-B, containing 700 mg/L of aluminum, is considered acceptable as the acceptance criterion for this type of certified materials is 100 ± 5 %. In addition, the accuracy level was of 0.61 % (CV). It is concluded that the analytical conditions optimization process to quantify Al in samples of Sprague Dawley rats using the flame AAS technique is acceptable in Al fortified samples, and from a certified reference standard.