Validation and application of a novel UHPLC‐MS/MS method for the measurement of furanodienone in rat plasma

A sensitive ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method was established to analyze furanodienone in rat plasma. In the process of chromatographic separation, selected reaction monitoring transitions for furanodienone and patchouli alcohol (internal standard, IS) were m/z 231.1 --> 83.2 and m/z 205.1 --> 95.1, respectively. Great linearity of furanodienone in plasma samples was found in the corresponding concentration range (r > 0.995). Intra- and inter-day precisions (RSD, %) were <11.3% in plasma, and the accuracy (RE, %) was within +/-10.7%. This method was used to the furanodienone study on rat pharmacokinetics after a single oral dose of 10 mg/kg of furanodiene. The results indicated that the maximum observed plasma concentration was 52.4 +/- 19.1 ng/ml at 1.2 +/- 0.7 h with an elimination half-life of 2.2 +/- 0.7 h. The obtained data indicated that furanodienone could be moderately distributed and eliminated.