Study of Variation on Fingerprint Chromatogram of Compatibility Extract Derived from Fructus Xanthii and Radix Astragali by HPLC-DAD

Objective To establish the method for analyzing compatibility components from Fructus Xanthii and Radix Astragali by HPLC fingerprint chromatogram and to study the influence of main characteristic peaks variations induced by compatibilities.Methods Diamonsil-C18 column(4.6 mm×250 mm,5 μm) was used to analyse these samples including the water extracts of Fructus Xanthii,Radix Astragali and compatibilities between them,with methanol and water containing 0.2% methanoic acid as mobile phases in a gradient elution,detection wavelength as 260 nm,and flow rate was 0.8 mL/min.Results HPLC fingerprint chromatogram of the compatibilities from Fructus Xanthii and Radix Astragali was established,and the whole characteristic peaks were the summation,with no new characteristic peaks existing.Compared with the single decoction,dissolution rate of some ingredients was decreased reciprocally in compatibilities.Conclusion The method is simple and convenient,which provides the references to clinical applications of compatibility and the basis for the quality assessment of compound.