Abstract B04: The effects of saliva exosome-like extracellular vesicles from locally advanced OSCC patients on OSCC cell lines

Microvesicles (MV) and exosomes (Ex) are extracellular vesicles (EV) differentiated by size and biogenesis pathway but sharing similar mechanisms of released and fusion with recipient cells. The EVs9 tumor cellular derived are implicated in mediating interactions with the cellular environment and are rich sources of biomarkers for noninvasive diagnosis, prognosis, and tumor monitoring. In this work we analyzed the effects of EVs like-exosomes derived from cell-free saliva of locally advanced and unresectable oral squamous cell cancer (OSCC) patients at different stages of disease progression: treatment naive (G1), after treatment and disease free for at least 2 years (G3), and healthy volunteers (G0). EVs were isolated by ultracentrifugation and analyzed by transmission electron microscopy (MET) and Nanosight quantification. The EVs effects on proliferation and migration upon SCC-4 and SCC-25 cell lines were investigated by MTT and scratch-wound assays. EVs exosomes-like were detected in saliva of all groups analyzed without differences in quantity and size distribution. The motility of the SCC-4 cell line was significantly modulated by the vesicles of the different groups analyzed after 12 h of treatment (P Citation Format: Karina A. Bispo, Fabyane O.T. Garcia, Tarcia A.C.B. Carvalho, Milena P. Mak, Gilberto de Castro Jr, Fatima S. Pasini. The effects of saliva exosome-like extracellular vesicles from locally advanced OSCC patients on OSCC cell lines [abstract]. In: Proceedings of the AACR International Conference held in cooperation with the Latin American Cooperative Oncology Group (LACOG) on Translational Cancer Medicine; May 4-6, 2017; Sao Paulo, Brazil. Philadelphia (PA): AACR; Clin Cancer Res 2018;24(1_Suppl):Abstract nr B04.