Compact, easy-to-use adaptive optics module for light-sheet structural and functional imaging of neuronal structures

We report a compact adaptive optics (AO) module with optimized optical design and photon budget, compatible with multiple wavelengths, and adaptable to most of existing Light-Sheet setups, enabling a 2 to 3-fold signal improvement on neuronal structures of the live, non-clarified drosophila brain (neurons, projections), at depths ranging from 50 to 100µm. We report similar signal improvement brought by AO on functional signals from neurons of the drosophila circadian clock network. The proposed setup paves the way to fully automatized AO Light-Sheet systems usable in routine by biologists.