Rational design of sulfidogenic granular sludge reactor with Clostridia as dominant bacteria for energy-efficient sulfate-laden wastewater treatment

Abstract The sludge flotation and washout are frequently observed in anaerobic sulfidogenic reactor. This challenge raised the interests of re-thinking/re-designing of a compact and low-flotation bioreactor. The present study investigated to understand the temporal dynamics of microbial community and granular sludge properties in a pneumatic-mixing reactor treating sulfate-laden wastewater. The findings revealed that the reactor performance and sludge properties were dynamically changed and correlated over long-term run. In the bioreactor, a rarer type of sulfate reducing bacteria (genus Clostridium XVIII) was remarkably enriched (∼30% abundance). The Clostridium XVIII-mediated COD removal (92.7±3.9%) was further confirmed via mass balance which demonstrated the growth rate of total active biomass and sulfate-reducing active biomass were 19.95 and 6.0 mg-COD/Linfluent respectively. The PICRUSt data suggested that i) high abundance of carbohydrate metabolism and S-reductase enzymes enriched, and ii) energy metabolism enzymes decreased which implies that the new SRB communities are more energy-efficient than conventional ones.