Cloning and prediction of B cell epitopes for F1L gene of orf virus.

In order to study F1L gene of orf virus(ORFV) ,the 1011bp-F1L gene of ORFV was amplified by PCR,and the PCR product was cloned into pMD18-T Easy vector.Then the purified F1L gene was subcloned into the pGEX-6P-1 vector and the recombinant plasmid was identified by PCR and restriction enzyme analysis.The authenticity and orientations of the gene were confirmed by sequencing.Results showed that the recombinant expression vector containing the ORFV F1L gene was successfully constructed.Bioinformatics analysis of the F1L gene showed that the F1L protein had hydrophilicity without signal peptide,and its B cell epitopes were located in 4 to 8,16 to 22,32 to 53,59 to 73,82 to 99 and 123 to 133 amino acids.The study laid the foundation for the development of methods for detection of ORFV,the preparation of monoclonal antibody against ORFV and the development of peptide vaccines against orf.