Effect of Pinus massoniana needle extract on root dentin demineralization in vitro

: 目的 评价马尾松松针提取物（PMNE）抑制根面牙本质脱矿的效果。方法 将根面牙本质块根据pH循环中所用实验溶液随机分为去离子蒸馏水（DDW）组，氟化钠（NaF）组，4%、8%和12%PMNE组，试件进行为期8 d的pH循环。显微CT选区测定各组未脱矿牙本质和脱矿牙本质的牙本质矿物密度（DMD）及两者DMD差值（ΔDMD），扫描电镜下观察pH循环后牙本质表面形貌。结果 PMNE各组和NaF组ΔDMD均显著低于DDW组（P 0.05），均显著低于4%PMNE组，高于NaF组（P 0.05), both of which were lower than the ΔDMD in the 4% PMNE group and higher than that in the NaF group (P<0.05). The dentin tubules were partly opened in the PMNE groups. The opening degrees of the dentin tubule in PMNE groups were significantly less and smaller than the opening degree in the DDW group and were larger than that in the NaF group. CONCLUSIONS: PMNE can inhibit the deminera-lization of root dentin and can slow down the reduction in DMD. PMNE has the potential to prevent caries, and 8% PMNE can effectively inhibit dentin demineralization.