Screening of Amylolytics and Cellulolytic Microbial with Potential use in Bioconversion of Cassava Solid Waste into Intermediate Fermentation Product

Microorganisms associated with cassava solid waste (CSW) were screened to hydrolyze cassava starch and cellulose. The cassava solid waste-by product of cassava processing obtained from three different factories in Gaya Baru Lampung Indonesia were investigated as sample. Liquid-enrichment media contain yeast extract (0.1 g/L) peptone (0.1 g/L) and dextrose (0.1 g/L) were employed as medium cultivation to propagate the indigenous microbial associated with diluted 100 g/L of sample. Screening of amylolitic and cellulolytic microbial were evaluated by clear zone measurement of hydrolysis starch and cellulose in agar medium assay. The highest colony of microbial counting were 7.8 x 104 cfu/mL and 5.6 x 105 cfu/mL for bacteria and fungi respectively. Among the isolates, two strains of bacteria namely bCSWa-3 and bCSWa-7, and two strains of fungi namely fCSWa-2 and fCSWa-5 have higher amylase activity than others. Furthermore, bacteria strains of bCSWc-9 and bCSWc-17, and fungi strains of fCSWc-7 and fCSWc-12 are the strains which have the higher cellulose activity than others. Selected strains will be applied in bioconversion of CSW into glucose as intermediate fermentation product to meet with valorization of SCW.