Expression of BtpB and BtpA versions in yeast and in inhibition of yeast endocytosis by the TIR domain of BtpB.

Western blotting of YPH499 cells extracts bearing the indicated BtpA (A) and BtpB (B) versions from pYES2-GFP plasmid derivatives, using antibodies anti-GFP (upper panels), anti-P-MAPK to show dual phosphorylation of Slt2 yeast MAPK and anti-G6PDH as loading control (lower panels). (C) Normarski and fluorescence microscopy of YPH499 cells expressing GFP-BtpB, GFP-BtpB-N and GFP-BtpB-TIR after 4h induction, stained with the endocytic marker FM4-64 for 1h. Scale bars indicate 5 μm. (PDF)