Effect of Weightlessness on Mouse NK Cell Bio-Activity in vitro

NK cell culture system in weightlessness was set up to observe cytotoxicity changes under the circumstances.The NK cells isolated from C57BL/6 mouse's spleen were collected from normal culture in 1G and weightlessness culture(wc) in rotating cell culture system(RCCS) respectively two days after the wc.Cytotoxicity of YAC-1 targets was detected with MTT assay.The transcription levels of perforin,granzyme B were examined with RT-PCR.The results showed that as compared with NK cells in normal control,their cytotoxicity cultured in weightlessness decreased significantly(P0.01).And their transcription levels of perforin,granzyme B cultured in weightlessness were much lower than those in normal control. Therefore,the weightlessness NK cells culture system in vitro was successfully established,and their cytotoxicity significantly decreased as compared with the normal control.