Wild-Type MIC Distributions and Epidemiological Cutoff Values for Selected Triazole and Echinocandin Antifungal Agents for Six Uncommon Species of Candida as Determined by CLSI Broth Microdilution Methods

Methods: 819 invasive clinical isolates of CD (70 isolates), CGU (198), CKF (112), CL (280), COR (102), and CPL (57) were collected from 2001-2010 worldwide (653 isolates from ARTEMIS and 166 from SENTRY). Susceptibility testing against six compounds using CLSI broth microdilution (BMD; 24-h incubation) was performed. Isolates were identified by standard biochemical methods and/or molecular sequencing. Results: The modal MICs (in µg/ml) for ANF,CSF, FLC, PSC, and VRC, respectively, were as follows: for CD (0.03, 0.06, 0.25, 0.03, 0.008), CGU (2, 0.5, 2, 0.12, 0.06), CKF (0.06, 0.015, 0.25, 0.06, 0.008), CL (0.5, 0.25, 0.5, 0.06, 0.008), COR (1, 0.12, 0.5, 0.03, 0.015), and CPL (0.015, 0.015, 2, 1, 0.12). The ECVs are summarized in the Table but could not be determined for CPL versus ANF.