The study on the effects and Mechanism of CS-PAA-EPI polymer magnetic nanopaficle on human bladder cancer T24 cells

Objective To investigate the effects and mechanism of CS-PAA-EPI polymer magnetic nanopaticle on human bladder cancer T24 cells. Methods Magnetic CS-PAA-EPI Nanopaticle was composed by CS,acrylic monomer (AA), EPI and Fe3O4 nanopaticle. The growth inhibiting rate of T24 cells were detected by the MTT techniche, apoptosis of T24 cells were observed by flow cytometry in vitro. The protein expression of T24 cells were tested by Western blotting techniche. Results To compare with control group, the growth inhibiting rate (76.6%, 71.8%, 68.4%) and apotosis rate (55. 13% ) of T24 cells in experimental group were significantly increased ," apoptosis peak" was observed in sub-G1 of experimental group cells. Conclusion CS-PAA-EPI Polymer Magnetic Nanopaticle can effectively kill human bladder T24 cells, the potential mechanism is cell apotosis. Key words: Chitosan; Apoptosis; Nanopaticle; Magnetic field