Selection and verification of a resistant starch-linked SSR marker in wheat.

Resistant starch(RS) is the sum of starch not absorbed by amylase in the small intestine.It's the focus of carbohydrate researches recently,and the unique physical function of RS has aroused many researchers' concern.The development and exploitation of RS molecule marker technology will reduce the cost and workload of RS determination,overcome the blindness and raise the efficiency of selection,provide theoretical basis to the breeding of wheat varieties with high RS content,and build the foundation of molecular marker-assisted selection of these varieties.In our experiment,M344 and wuchun3 whose RS content were significantly different were used as parents to develop F2 generations.A SSR marker Xbarc 59 conferring high resistant starch content was screened by bulked segregant analysis(BSA) method.In F2 generation of M344/wuchun 3,SSR primer Xbarc 59 is closely correlated to RS content,and the amounts of RS is significantly different at 1% lever among the three genotypes of high-parent type,heterozygote and low-parent type.The average content of higher and lower RS individuals differentiated by Xbarc 59 was 1.44% and 0.99%,respectively,and the difference is significant at 0.01 level.So the primer Xbarc 59 could be an available molecule marker to select the wheat breeding materials with high RS content.