Dietzia psychralcaliphila sp. nov., a novel, facultatively psychrophilic alkaliphile that grows on hydrocarbons

A novel, facultatively psychrophilic alkaliphile that grows on a chemically defined medium containing n-alkanes as the sole carbon source was isolated from a drain of a fish product-processing plant. The isolate was an aerobic, non-motile, gram-positive bacterium. The bacterium was catalase-positive and oxidase-negative. The cell wall contained meso-diaminopimelic acid, arabinose and galactose; the glycan moiety of the cell wall contained acetyl residues. The G+C content of the DNA was 69.6 mol %. Phylogenetic analysis based on 16S rRNA gene sequences showed that the isolate was closely related to members of the genus Dietzia (96.1-96.8% similarity). Comparisons of phenotypic and chemotaxonomic characteristics between the isolate and the two known Dietzia species showed that they were very similar. However, the isolate differed from the two known Dietzia species in growth temperature range and certain physiological characteristics. DNA-DNA hybridization revealed that the isolate had 38.4 and 49.7% relatedness, respectively, to Dietzia maris and Dietzia natronolimnaea. On the basis of the physiological and biochemical characteristics, the phylogenetic position as determined by 16S rRNA gene analysis and DNA-DNA relatedness, it is concluded that the isolate should be designated as a novel species, for which the name Dietzia psychralcaliphila sp. nov. is proposed. The type strain is ILA-1T (= JCM 10987T = IAM14896T = NCIMB 13777T).