Identification of lactic acid bacteria as antimicrobial from milk Toraja Belang buffalo

Lactic acid bacteria can produce antimicrobial compound. The bacteria can be found in milk. The objective of this research is to study antibacterial activity and identification of lactic acid bacteria from milk of Toraja Belang Buffalo South Sulawesi. The lactic acid bacteria were isolated by pour plate method using MRSA containing 1 % CaCO3. Antimicrobial activity of the isolates against Salmonella typhi, Staphylococcus aureus and EPEC were assayed using agar well diffusion method. The result showed that among 14 isolates of LAB, the most potent antibacterial activity of Cell Free Supernatant against to EPEC is K1B, Staphylococcus aureusis K4A2 and Salmonella typhi is K5B2. Based on the 16S rDNA sequence, K1B, K4A2 and K5B2 isolates were identified as Enterococcus faecalis ATCC19433T with similarity 99.758 %, 100% and 99.759% respectively.