Expression of G-protein coupled receptors in Escherichia coli for structural studies
2010
To elaborate a high-performance system for expression of genes of G-protein coupled receptors (GPCR), methods of direct and hybrid expression of 17 GPCR genes in Escherichia coli and selection of strains and bacteria cultivation conditions were investigated. It was established that expression of most of the target GPCR fused with the N-terminal fragment of OmpF or Mistic using media for autoinduction provides high output (up to 50 mg/liter).
Keywords:
- Correction
- Source
- Cite
- Save
- Machine Reading By IdeaReader
45
References
26
Citations
NaN
KQI