The effect of compressive force combined with mechanical vibration on human alveolar bone osteoblasts

Abstract Objective This study aimed to investigate the effects of compressive force combined with mechanical vibration on the expression of pro-inflammatory cytokines that promote osteoclastogenesis and related to orthodontic tooth movement acceleration in human alveolar bone osteoblasts in vitro . Methods Osteoblasts were subjected to compressive force (C), mechanical vibration (V), compressive force combined with mechanical vibration (CV), or no force as a control for 12, 24 and 48 h. Interleukin-1 beta (IL-1β), interleukin-6 (IL-6), receptor activator of nuclear factor kappa-Β ligand (RANKL) and osteoprotegerin (OPG) mRNA and protein expression were assessed using quantitative real-time polymerase chain reaction and enzyme-linked immunosorbent assays. Results In C and CV groups, IL-1β and IL-6 mRNA and protein expression were significantly higher and OPG mRNA and protein expression were significantly lower than control and V groups. However, the expressions were not different between C and CV groups. RANKL mRNA and protein expression were not different between any groups. While, OPG mRNA and protein expression in V group were significantly higher than control group. Conclusions Vibration neither enhanced nor inhibited the expression of IL-1β, IL-6, RANKL and OPG in compressed human alveolar bone osteoblasts.