Effects of plant growth substances on callus re-differentiation of medicinal plant Achyranthes bidentata

In this study, callus from stem could easily differentiate to form bud and root, while re-differentiation potential of callus from leaf and petiole was lower, especially bud was not discovered in callus from petiole. Compared with other plant growth substances, effect of 2,4-D on callus re-differentiation of various explants was extremely significant, simultaneously the interaction among plant growth substances was also discovered, in which the optimum combination of plant growth substances for bud and root formation from callus of stem was respectively as follows, combination of 0.5mg/L 2,4-D, 1.0mg/L NAA, 0.1mg/L IBA and 0.1mg/L ZT, and combination of 0.5mg/L 2,4-D, 1.0mg/L KT, 0.5mg/L NAA and 0.5mg/L IBA. Besides, combination of plant growth substances for root formation from callus of leaf and petiole was also obtained. In brief, the re-differentiation capacity of callus from various explants was different, and roles of plant growth substances in re-differentiation were also significantly diverse, thus these results would provide theory basis for tissue culture and plant regeneration of Achyranthes bidentata.