Screening of differentially expressed gene in HIV/HCV co-infected patients

Methods Eligibility Criteria including 20-40 year-old male, similar social-economic background, treatment naive, same transmission route methods (Sex or Injection drug), and CD4 count >200 cells/mm. All research objects signed on informed consent. The project proceeded after approval of the local REC. Human blood samples were collected from 13 HIV/ HCV coinfected patients, 8 mono infected patients, and 8 normal control. At first, CD4 and CD8 positive T cells were separated with magnetic activated cell sorting, then followed by RNA extraction. After reverse transcripted to cDNA with anchor primer and amplified with random primers, the products were run on PAGE electrophoresis followed by silver stain. The bands with different expression level were extracted from gel and cloned for sequencing. The expression changes were confirmed by SYBR Green real-time quantitative PCR.