Multi-spectral scanning fiber endoscope with concurrent autofluorescence mitigation for enhanced target-to-background ratio imaging
2014
We developed a multispectral fluorescence-reflectance scanning fiber endoscope (SFE) for wide-field molecular imaging
of fluorescence-labeled molecular probes. Concurrent multi-channels imaging with the wide-field SFE also allows for
real-time mitigation of background autofluorescence (AF) signal, especially when the FDA approved fluorescein is used
as the target fluorophore. In the current study, we demonstrated a real-time AF mitigation algorithm on a tissue phantom
which featured molecular probe targeted cells of high grade dysplasia on a substrate containing AF species. The targetto-
background ratio was enhanced by over an order of magnitude when applying the real-time AF mitigation algorithm.
By minimizing the background signal, multispectral fluorescence imaging can provide sufficient image contrast and
quantitative target information for detecting small pre-cancerous lesions in vivo.
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