Use of a lytic enzyme system from Cytophaga sp. in the lysis of Gram-positive bacteria

Abstract A lytic enzyme system from Cytophaga sp. has been used for lysis of the Gram-positive bacteria , Bacillus and Corynebacterium. The optimum pH and temperature for the lytic reaction were 9.2 and 50°C, respectively. The effect of substrate and enzyme concentration have also been studied. Protein release was followed and the potential of using bacteriolytic enzymes for large-scale cell lysis and release of intracellular material is discussed .